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KMID : 0352720130370020248
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Journal of Ginseng Research
2013 Volume.37 No. 2 p.248 ~ p.253
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HPLC-based metabolic profiling and quality control of leaves of different Panax species
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Yang Seung-Ok
Lee Sang-Won
Kim Young-Ock
Sohn Sang-Hyun
Kim Young-Chang
Hyun Dong-Yoon
Hong Yoon-Pyo
Shin Yu-Su
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Abstract
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Leaves from Panax ginseng Meyer (Korean origin and Chinese origin of Korean ginseng) and P. quinquefolius (American ginseng) were harvested in Haenam province, Korea, and were analyzed to investigate patterns in major metabolites using HPLC-based metabolic profiling. Partial least squares discriminant analysis (PLS-DA) was used to analyze the the HPLC chromatogram data. There was a clear separation between Panax species and/or origins from different countries in the PLS-DA score plots. The ginsenoside compounds of Rg1, Re, Rg2, Rb2, Rb3, and Rd in Korean leaves were higher than in Chinese and American ginseng leaves, and the Rb1 level in P. quinquefolius leaves was higher than in P. ginseng (Korean origin or Chinese origin). HPLC chromatogram data coupled with multivariate statistical analysis can be used to profile the metabolite content and undertake quality control of Panax products.
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KEYWORD
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Panax leaves, Ginsenosides, HPLC, Metabolic profiling, Quality control,
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